Blastocyst Transfer

On the day of the ovum pick up make center well with cleavage medium and kept overnight in the incubator.
The next day (day 1) after the denudation (IVF embryos) and PN assessment is done (IVF and ICSI embryos) the selected embryos are put in the culture medium
This is done with a 170um flexi pipette.
The dishes are then put in the incubator at 37*C and 6% Co2.
On Day 2 a dish with cleavage media is made in the same way and kept in the incubator. The embryos in the cleavage media are assessed for cleavage and graded.
On day 3 the embryos from culture media are then tranferred into well containing fresh culture medium.
The embryos are generally at the eight cell stage.
On day 4 the embryos are not touched but a transfer dish (0.8 ml ml G2.2 media in center well) is prepared for the blastocyst transfer the next day.
On day 5 the embryos are assessed. The blastocyst from embryos with high PN scores on Day 1 are given priority.
The blastocyst are washed in a wash dish, this time a 300um pipette is used and then transferred to the final Embryo transfer dish.